De novo assemblies were generated using CLC Genomic Workbench, Trinity and Velvet/Oases with the best overall contig performance resulting from the CLC assembly. We obtained 12,177,770 reads after trimming and processing with an average length of 97 bp. Developing genomic resources for this species can facilitate the production of improved culture lines that can withstand raised salinity conditions, and so we have applied high-throughput Ion Torrent sequencing of transcriptome libraries from six target osmoregulatory organs from striped catfish as a genomic resource for use in future selection strategies. The culture industry is facing a significant challenge however from saltwater intrusion into many low topographical coastal provinces across the Mekong Delta as a result of predicted climate change impacts. The Workbench is now ready to use.Striped catfish (Pangasianodon hypophthalmus) is a commercially important freshwater fish used in inland aquaculture in the Mekong Delta, Vietnam. You can now close the Workbench and then start it up again as a standard user. Launch the plugin manager and install any plugins you downloaded earlier by clicking on the Install from File button. If it does not, then you can launch it yourself by choosing the menu option:ġ0. The License Manager of the Workbench should start up automatically when you start up the Workbench. To locate an existing license file from a Workbench installed to a different area of the machine, look in the folder called licenses for that other installed Workbench copy. If you have downloaded a license file (step 3) or you have chosen to install a minor update to a new location on your machine rather than the existing installation location, then install the license file by choosing the option Import a license from a File within the License Manager. Once the software is installed, start up the Workbench as an administrative user.ĩ. Run the Workbench installer as an administrative user on the non-networked machine.Ĩ. This would usually be done by saving to an area accessible to that machine, or by saving the files to a USB stick or other form of portable storage available to you and loading them onto a non-networked machine.ħ. Transfer the Workbench installer, plugin files, and the license file to the non-networked machine you wish to install the software on. If you are upgrading the Workbench with another version in the same release line, then you do not need to download a license file. As an example, v20.0.x and 20.0.4 are in the same major release line, as both have "20" as the first digit, and they can be run using an existing license file that supports the 20.x line.Ħ. That information is written for the QIAGEN CLC Genomics Workbench but is relevant for other CLC Workbenches also. Information about how to accomplish this can be found at:ĥ. ![]() 12.x -> 20.x), then you will need to download a license file for the (non-networked) machine you plan to install the Workbench on. You will need your license order ID and the host ID of the machine the software will be run on for this. If you are installing this software for the first time or are upgrading to a new major version (e.g. The relevant plugin files can be downloaded by clicking the button in the Download column on that page.Ĥ. If you wish to install any of the available Workbench plugins, please download the Workbench plugin files from the plugin page. How do I install or upgrade my Workbench on a non-networked machine?ĬLC Workbench software can be installed on a machine that is not connected to the external network by doing the following:ġ. Using a machine that is connected to the internet, download the installer file for the CLC Workbench.Ģ. Information about how to download the installer file for your Workbench can be found in the related FAQ: Where can I get installer files for QIAGEN CLC software?ģ.
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