De novo assemblies were generated using CLC Genomic Workbench, Trinity and Velvet/Oases with the best overall contig performance resulting from the CLC assembly. We obtained 12,177,770 reads after trimming and processing with an average length of 97 bp. Developing genomic resources for this species can facilitate the production of improved culture lines that can withstand raised salinity conditions, and so we have applied high-throughput Ion Torrent sequencing of transcriptome libraries from six target osmoregulatory organs from striped catfish as a genomic resource for use in future selection strategies. The culture industry is facing a significant challenge however from saltwater intrusion into many low topographical coastal provinces across the Mekong Delta as a result of predicted climate change impacts. The Workbench is now ready to use.Striped catfish (Pangasianodon hypophthalmus) is a commercially important freshwater fish used in inland aquaculture in the Mekong Delta, Vietnam. You can now close the Workbench and then start it up again as a standard user. Launch the plugin manager and install any plugins you downloaded earlier by clicking on the Install from File button. If it does not, then you can launch it yourself by choosing the menu option:ġ0. The License Manager of the Workbench should start up automatically when you start up the Workbench. To locate an existing license file from a Workbench installed to a different area of the machine, look in the folder called licenses for that other installed Workbench copy. If you have downloaded a license file (step 3) or you have chosen to install a minor update to a new location on your machine rather than the existing installation location, then install the license file by choosing the option Import a license from a File within the License Manager. Once the software is installed, start up the Workbench as an administrative user.ĩ. Run the Workbench installer as an administrative user on the non-networked machine.Ĩ. This would usually be done by saving to an area accessible to that machine, or by saving the files to a USB stick or other form of portable storage available to you and loading them onto a non-networked machine.ħ. Transfer the Workbench installer, plugin files, and the license file to the non-networked machine you wish to install the software on. If you are upgrading the Workbench with another version in the same release line, then you do not need to download a license file. As an example, v20.0.x and 20.0.4 are in the same major release line, as both have "20" as the first digit, and they can be run using an existing license file that supports the 20.x line.Ħ. That information is written for the QIAGEN CLC Genomics Workbench but is relevant for other CLC Workbenches also. Information about how to accomplish this can be found at:ĥ. ![]() 12.x -> 20.x), then you will need to download a license file for the (non-networked) machine you plan to install the Workbench on. You will need your license order ID and the host ID of the machine the software will be run on for this. If you are installing this software for the first time or are upgrading to a new major version (e.g. The relevant plugin files can be downloaded by clicking the button in the Download column on that page.Ĥ. If you wish to install any of the available Workbench plugins, please download the Workbench plugin files from the plugin page. How do I install or upgrade my Workbench on a non-networked machine?ĬLC Workbench software can be installed on a machine that is not connected to the external network by doing the following:ġ. Using a machine that is connected to the internet, download the installer file for the CLC Workbench.Ģ. Information about how to download the installer file for your Workbench can be found in the related FAQ: Where can I get installer files for QIAGEN CLC software?ģ.
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![]() California county’s cannabis industry on ‘the brink of irreversible failure’.SF tech firm DocuSign conducts layoffs again, continuing worrisome trend.Guy Fieri-approved California restaurants on the way to Lake Tahoe.Iconic Santa Cruz landmark to be destroyed.Mountain lion tears dog on leash away from its owner in California neighborhood.Horoscope for Saturday, 2/18/23 by Christopher Renstrom.Elon Musk has late-night Twitter outburst, blasting OpenAI, staff leaks. ![]() The "Full House" home at 1709 Broderick St. Ramsdell noticed that the comments fall into three different camps: positive responses, critical ones pointing out the possibility that Uncle Jesse was indeed expelled for the motorcycle stunt and people simply asking what the hell a PowerPoint party is. In the five days since posting the video, it has gone viral with 114,000 views, and an associated Reddit post has 11,000 upvotes and 1,100 comments. I was like, 'no way, this is the jackpot,'" Ramsdell said in a phone interview. That he was down to talk to me, and that he remembered, and so specifically, I was out-of-my-mind happy. To Ramsdell's surprise, Burkhard not only wrote him back but knew about this exact plot hole, which a script assistant pointed out during filming but considered too insignificant to correct. Rather than just settle for pointing out the inconsistency, Ramsdell took it a step further, contacting the show's writer, Tom Burkhard. Two seasons later, in the episode "Educating Jesse," he admits to the rest of the family that he was a high school dropout, seemingly contradicting the episode that had aired two years earlier. A flashback shows him riding a motorcycle into the school gym and joking about how he couldn't be expelled a day before graduation. In the ninth episode of the fourth season, which aired in November 1990, Uncle Jesse practices a few songs to play with his old band at his 10-year high school reunion. The specific plot point in question revolves around Uncle Jesse's educational background. He told SFGATE that the video took 80 hours to produce. The project was based on a slideshow he presented at a pandemic PowerPoint party thrown by a friend in January 2021. "Every day, there was this magical hour between school and homework, where I would get to spend two marvelous episodes in San Francisco with the Tanners," he narrates at the start of the 11-minute video. On November 4, Paul Ramsdell, a New York-based TV producer, released a YouTube video titled "House Full of Lies." It delves deep into a specific plot point that Ramsdell has obsessed over since childhood, when he'd spend afternoons rewatching the show in his basement after moving to a new town. ![]() What's more, it totally free for users, without any adware, spyware or limitations.Ħ. And you will find it is very easy to operate on computer, interface will guide you to finish all steps you need to do. It can burn any video into DVD dis, the program supports over 45 files formats, over 60 video codecs, over 40 audio codecs, it is means that you can back up and protect almost of your files into DVD disc.Įxcept formats of files, there are more functions will make you happy, with this free DVD maker app, you can add a menu and subtitle on DVD disc you want to burn, it is very useful but rare on a lot of free DVD creators. 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So, you will not need to change the format of video before creating a DVD disc, it is really convenient.Ĥ. Actually, it supports almost formats of video, such as MP4, AVI, MOV, etc. Of course, you can also make photo slideshow by DVD Styler, it is a very wonderful method to keep important photos.Īs a free DVD creator, DVD Styler is totally free but powerful. You are allowed to burn DVD videos with interactive menus, add subtitle and audio on your DVD disc. When you are looking for a giant and free DVD creator for your videos, DVD Styler can offer you a lot of functions to create a DVD disc. DVD Styler – Free DVD Creator for Windows Of course, it supports widest range of media formats.Įxcept burning DVDV disc as a DVD creator app, Free Burning Studio can also create disc ISO image with premium quality, sometimes you might want to make ISO files from DVD disc, you can choose Free Burning Studio. If you are going to burning DVD disc or back up date, you can have a try with Free Burning Studio, it can easily and effectively burn audio. With a great free DVD creator, you can do a lot of things if you use DVD usually, such make DVD disc, keep your personal date etc. Free Burning Studio – Free DVD Creator for Windows 10 It supports AVI, MP4, MKV, MPEG, VOB and FLV etc.Ģ. If the controlling system of your computer is Windows10, it is also an all-in-one program. ![]() On the other hand, you can use it to add chapter titles, subtitles, even organization through the creation of contents pages. ![]() So, you don't need spend a long time to wait during burning. No matter what purpose you want to make, personal library or professional DVD disc for sale both are OK with WinX DVD Author. WinX DVD Author – Free DVD Creator for WindowsĬreating DVD disc on Windows10, WinX DVD Author will be the first one free DVD creator you have to choose. Part 2: Top 9 Free DVD Creator apps you really want 1. On the interface, import and edit the video files, edit audio track and subtitle, and then start to create the DVD disc. Insert a blank DVD disc into your computer. "enhanced angry vanilla bots.vpk" = this vpk can be used with most bot mods except "Left 4 Bots" (there are some unknown errors on console though normally it's supposed to work as I used different files to trigger the scripts).Ģ. There are 2 vpk files in the zip based on the usage:ġ. This mod works together with other bot mods ![]() CLEAN THE WINCH every six months or whenever it is dirty. ![]() Business is a locally owned and operated franchise that specializes in carpet cleaning. In most situations, the law permits, but does not require, a judge to expunge an eviction case from the courts records. Keep all unnecessary personnel away from winch while in operation. Ensures that the restaurant follows all cash control and security procedures (e.g. Browse businesses for sale in Winona County, MN on BizBuySell.Ensures preventative maintenance of restaurant facility and equipment is completed in accordance with Company standards, interacting with external vendors as required.Maintains and regularly monitors a list of all restaurant assets.West Virginia State Troopers, Greenbrier County Sheriff’s Deputies, and Lewisburg Police Officers arrested a total of 19 drivers on Friday, April 2, 2021. Places and receives inventory truck orders LEWISBURG, WV (WVNS) Law enforcement agencies in Greenbrier County arrested multiple people and seized drugs as part of Operation: Spring Cleaning.Manages inventory costs and maintains inventory by performing Daily, Weekly, and Monthly inventory inspections.You submerge the parts to be cleaned into the tanks cleaning solution. The AM operates under the direction of the RGM and directly manages a team of Hourly Shift Coordinators and Team Members (20-45 employees). The Hot Tank is an immersion cleaning method. ![]() The AM supports the Restaurant General Manager (RGM) in managing the daily operations of a single restaurant, driving key performance outcomes. strength: N/A: Operation Spring Cleaning is an expedition that escalates from Angel Fortress and consists of 4 parts. The Assistant Manager (AM) is the operations leader of the restaurant focused on profitability, Guest service, people development, and operations management. Operation Spring Cleaning Type: Expedition: Rating: Unrated: Found in: Null: Max ship size: Unknown: Faction: Angel Cartel: Damage to deal: Ex Kin: Damage to resist: 62 Ex 22 Kin: Sig. ![]() There is an exit tax for people severing residential ties to Canada and “all realized and unrealized gains to be reported for digital and crypto assets,” says Mr. But, he cautions, “if you’re claiming losses, you’re more likely to be audited.” Definitely claim them, just keep clear records, he says. Sedigh says traders who lose money on crypto can claim their losses in the tax year they occurred, applying them to their income to get a tax break. Beaudry says that if the trader “carries on the activity for commercial reasons and in a commercially viable way undertakes activities in a businesslike manner, which might include preparing a business plan and acquiring capital assets or inventory promotes a product or service or shows that they intend to make a profit,” their profits could be considered business income. It depends on factors including how speculative the trades are, if trading occurred on borrowed funds, the trader’s level of expertise, how long the position was held and the number of trades per month, he says.ĬRA’s Ms. The way CRA determines whether someone’s crypto trading is a business isn’t an exact science. Traders who realize capital gains only pay tax on half the profits, while those earning business income pay tax on the full amount, Mr. Some profits are capital gains, some are business income “Reports should be drawn on a monthly basis as some trading platforms have been known to lose data or shut down,” he adds. He recommends converting each trade to Canadian dollars when it occurs and logging those numbers. Sedigh, which means gains and losses need to be calculated. Sedigh – a partner in Toronto-based Triple M Professional Corp., which specializes in accounting for cryptocurrency buyers – says he’s seen a huge influx in people seeking his services since the start of the pandemic.Īll crypto transactions are taxable eventsĪ taxable event is triggered each time a crypto-to-crypto transaction happens, says Mr. That’s a common misunderstanding, says chartered professional accountant Mehran Sedigh, who says any trade is a taxable transaction, and traders can be fined if the Canada Revenue Agency discovers a taxpayer’s crypto earnings before they are reported voluntarily. Wallace says she hasn’t given much thought to the tax repercussions of her relatively minor crypto purchase, saying she assumed it would only be taxable if she pulled it out of the exchange she uses for trading. ![]() “You only put in what you can lose,” she says, noting she’s saving what’s still invested as a potential nest egg for her preschool-age daughter. Wallace, 46, says she converted the amount of her initial investment back into Canadian dollars and is leaving the rest invested in crypto to see what happens. ![]() The sneakers were available in low-top (CN8608-002) and high-top (CN8607-002) versions, both showing off the iconic Dior monogram on the Swoosh. Of course, nothing has proven as popular as the 2020 Dior Air Jordan 1 collaboration, the brand's first foray into an athletic crossover. Styles like the B23 in 2019 gave the brand a mix of styles that highlighted Dior's luxury status. While Dior had shoe creations, such as the B17 or the older Sweat, it was the design hand of Kim Jones that helped put Dior on the collectible sneaker map. When Dior died of a heart attack at the age of 52, a line of artists and designers took over the fashion label.ĭior's place in sneaker culture has advanced significantly since the late 2010s. By 1953, Dior had enlisted designer Roger Vivier's help to create a line of women's shoes, and a young Yves Saint Laurent joined him in 1955. The following year, his creations grew, with a New York City store opening on Fifth Avenue. He launched his first collection in 1947 from his Paris fashion house in 1947, focusing on women's designs. He turned to style as his main interest and worked for other designers before striking out on his own in 1946. We send the most important news directly to your cell phone every day.Christian Dior introduced a "new look" for fashion upon his 1947 collection release, embracing full forms of structured designs for women's dresses that brought lavishness back to style following World War II.īefore entering the world of fashion, Dior ran a French art gallery. Never miss new sneaker releases, restocks or news again. The release is in the early months of 2022. Look forward to a great selection with exciting colourways from Dior and Cactus Jack. The first models from the Cactus Jack x Dior Spring/Summer 2022 collection have already been shared online. ![]() Cactus Jack x Dior Spring/Summer 2022 Collection It has also been confirmed that several colourways are dropping, ranging from earth-toned models to snakeskin-inspired sneakers. Thanks to the first images, we see a rather flat construction with levelled soles and an upper reminiscent of the sport’s shoes. Info is still thin to this point, but the sneakers are reportedly inspired by du skate culture. Now, to compete with the highly coveted Louis Vuitton x Nike collection, Dior has gotten the help of Travis Scott and his label Cactus Jack.ĭior’s lead shoe designer Thibo Denis has announced this big collaboration on Instagram, along with the title “B713” ahead of the official Spring/Summer ’22 show. With Nike, the label has already shown how coveted the luxury label can be outside its own range. The release details are still missing, but the B713 will supposedly drop with the 2022 summer collection.ĭior’s influence on the fashion world is huge. Below, suede stripes separate the upper from the bright midsoles with rubber outsoles, and the thick tongues are reminiscent of early skate shoes from the 2000s. The new version features different shades of blue, while the upper is made of premium leather at the collar and mesh at the midfoot. Thibo Denis shared the first images of the Cactus Jack x Dior B713 at Paris Fashion Week last month, and today, he shares detailed shots of another model. ![]() How the body processes (metabolizes) alcohol: People who need comparatively more alcohol to achieve an effect have a higher risk of eventually developing health problems related to alcohol.įor AUD to be diagnosed in the U.S., the individual must meet the criteria laid out in the Diagnostic and Statistical Manual of Mental Disorders (DSM), published by the American Psychiatric Association (APS).Alcohol advertising and media coverage of it may increase the risk by conveying the message that excessive drinking is acceptable. Media and advertising: In some countries, alcohol is portrayed as a glamorous, worldly, and cool activity.On the other hand, consuming too much alcohol may increase the risk of depression, rather than reducing it. Depression: People with depression may deliberately or unwittingly use alcohol as a means of self-treatment. ![]() Low self-esteem: Those with low self-esteem who have alcohol readily available are more likely to consume too much.This can eventually lead to alcohol-related problems. Peer drinking: People whose friends drink regularly or excessively are more likely to drink too much.If stress and anxiety levels are high, a person may consume alcohol in an attempt to blank out the upheaval. Stress: Some stress hormones are linked to alcohol misuse. ![]() The effect was found to be nearly two to four times that of other prevention strategies, such as school programs or media campaigns. One study registered a significant drop in alcohol-related deaths after one state raised alcohol taxes.
![]() TA and Petro truck stops offer truck maintenance services, fast-food options and 24-hour homestyle restaurants. The Love’s Connect app offers rewards for purchases made at Love’s or Speedco locations. Love’s provides fuel, tire care, mechanical services and travel essentials. Founded in 1964, Love’s has over 550 locations in 41 states. Their travel centers offer well-known fast-food providers, and the myRewards Plus app lets drivers earn free shower and drink credits. Professional drivers have access to certified CAT scales, fuel stations, truck washes, service centers, laundry and showers. With over 740 locations in 44 states, Pilot Flying J is the largest travel center operator in North America. Some of the largest truck stop chains include: As drivers earn points, they receive credit for showers, food and fuel. Most chains offer loyalty programs or rewards points that can be used at any location. Truck stop chains are dotted across the map. That’s a lot to choose from! Largest Truck Stop Chains in America Apps like Trucker Path track ratings for over 40,000 truck stops and travel centers in the United States and Canada. NATSO defines a “truck stop” as a location with at least one shower, 15 parking spaces and diesel available for sale.įrom chain truck stops to mom-and-pop establishments, the number of truck stops open in the United States is much higher. The National Association of Truck Stop Operators (NATSO) represents more than 2,000 truck stops and travel centers. We’ve compiled a list of popular truck stops in this article, including chains and independent businesses. Whether you’re stopping overnight, need to fuel up quickly or want a safe place to wait for severe weather to clear, truck stops are essential to every driver’s journey. Over your career, you’ll learn which truck stops are hidden gems and which you’d rather avoid at all costs. A good truck stop can make any trip better. They need to know where they will eat, shower and sleep. Need a shower? Need a coffee? Clean laundry? How about a place to park for the night?ĭrivers must think about the logistics of life on the road. Over-the-road drivers are on the road day in and day out. Thus, creating an enriching and engaging experience for app users resulting in raised user retention rates. Moreover, market players are incorporating smart technologies in their offerings which deliver personalized recommendations of meals and recipes. These factors are also supporting the market’s growth. One can easily email or share it through Facebook or other social media platforms to their near and dear ones right away. In addition to this, it has become easier to share recipes online that any individual or their family has loved. There are hundreds and thousands of recipes available online, which gives individuals the advantage of trying out new recipes and improvising without purchasing expensive cookbooks.īy providing a visual medium for cooking, recipe apps have been working wonders and thus driving market growth. It provides recipes from across the globe and hence serves as an excellent cookbook. In simple words, a food recipe app transforms the Smartphone into a cookbook. It is created as per a person’s diet and even shares amazing recipe tips and tricks all at our fingertips by just scrolling the applications on devices. Currently, the healthy recipe apps offer step-by-step instruction guides for a monthly breakfast plan. It has made life more effortless and convenient. These recipe apps are made available to the public in paid as well as free editions. ![]() Today, there are recipes for diverse cuisines such as Mexican, Italian, Chinese, Indian, Japanese, French, and others. Or even offer assistance to make a weekly meal plan that goes with their diet. These apps provide step-by-step instructions to help individuals prepare a hearty homemade meal. ![]() To make cooking all the more fun recipe apps have been designed for iOS and Android. To Get Detailed Analysis: Download Report PDF Global Recipe Apps Market Definition |
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